Optimization of supercritical CO2 extraction process of Magnoliae Officinalis Cortex and anti-oxidant activities of extracts / 中草药

Objective: To optimize the supercritical CO2 extraction process of active ingredients from Magnoliae Officinalis Cortex (MOC) and explore the antioxidant activity of the extracts. Methods: The content of magnolol and honokiol of the supercritical CO2 extracts of MOC was determined by HPLC, and the extraction process was optimized by orthogonal experiment. The antioxidant activities of the extracts were determined by MTT. Results: The optimum extraction pressure of magnolol was 25 MPa, the extraction temperature was 55 ℃, the amount of CO2 was 30 kg, and the optimum extraction parameters mentioned above of honokiol were 15 MPa, 50 ℃, and 25 kg, respectively. Conclusion: Under the optimum extraction conditions, magnolol and honokiol have high extraction efficiency, good repeatability, stability and feasibility, and the extract have good antioxidant activity.

Preliminary study on anti-oxidant activities and material base of n-butanol extract of Boschniakia rossica / 中草药

Objective To investigate anti-oxidant activities of the n-butanol extract of Boschniakia rossica (BEBR), and preliminarily determine the material basis of its antioxidant activities. Methods Antioxidant activities of different polarity extraction of BEBR were performed by using 1,1-dipheny-2-trinitrophenylhydrazine (DPPH), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), diammonium salt (ABTS) radical scavenging assays and ferric reducing anti-oxidant power as the index. The aging rats induced by D-galactose were used as models to investigate the effects of different n-butanol extract parts on the content of MDA and the activity of SOD and CAT in liver tissue; The chemical composition of BEBR was preliminarily analyzed by HPLC-DAD-ESI-MS/MS. Results BEBR had good scavenging effect on free radicals of DPPH and ABTS, and well performation in ferrous reduction experiments of FRAP determination. Compared with the model group, all indexes of MDA, SOD, and CAT in liver tissues of rats from different dose groups with BEBR were significantly improved (P < 0.01), which showed that levels of MDA reduced, the activity of SOD and CAT increased and back to above normal levels. The anti-oxidant activity in vivo showed in a dose-dependent manner. Nineteen chemical constituents were tentatively characterized by HPLC-DAD-ESI-MS/MS, of which 14 were phenylpropanoid glycosides (PPGs). Conclusion BEBR has good anti-oxidant effects and PPGs are the main active constituents.

Fatty acid composition, antioxidant and antibacterial properties of the microwave aqueous extract of three varieties of Labisia pumila Benth

BACKGROUND: The present study was conducted in order to evaluate the fatty acid profile, anti-oxidant and anti-bacterial activities from the microwave aqueous extract of the leaves of three different varieties of Labisia pumila Benth. RESULTS: The chemical analysis of the extract showed that fatty acids (palmitic, palmitoleic, stearic, oleic, linoleic and α-linolenic) acid as the main components in three varieties of L. pumila leaves. Furthermore, the obtained results of the anti-oxidant revealed that L. pumila var. alata contained higher anti-oxidative activities compared to var. pumila and var. lanceolata. However, these values were lower than the tested anti-oxidant standards. On the other hand, the aqueous leaf extracts in all three varieties of L. pumila were also found to inhibit a variable degree of antibacterial activities against eight bacteria (four Gram-positive and four Gram-negative bacteria). CONCLUSIONS: In this study, it was observed the leaves of three varieties of L. pumila exhibited variable patterns of fatty acids and the microwave aqueous extraction possess anti-oxidant and anti-bacterial activities.

Steroidal saponins and phenylic constituents from Lilium lancifolium and their anti-oxidant activities / 中草药

Objective: To study the steroidal saponins and phenylic constituents in the bulbs of Lilium lancifolium and their anti-oxidant activities in vitro. Methods: The constituents were isolated and purified by chromatographic technique and recrystallization, and their structures were identified by spectral methods together with physiochemical analyses. The anti-oxidant effects of these constituents on DPPH and ABTS were screened in vitro. Results: Eleven constituents were isolated including seven first-found ones (1-5, 9, and 10) and four known compounds in this plant. They were cis-1-O-p-coumaroylglycerol (1), trans-1-O-p-coumaroylglycerol (2), caffeoyl glyceride (3), 3,4- dihydroxybenzaldehyde (4), salicylic acid (5), (25R,26R)-26-methoxyspirostan-5- ene-3β-O-α-L-rhamnopyranosyl-(1→2)-[β-D-glucopyranosyl- (1→6)]-β-D-glucopyranoside (6), (25R,26R)-3β, 17α-dihydroxy-26-methoxyspirostan-5-ene-3β-0-a-Z.-rhamnopyranosyl-(l- >2)-[β-D-glucopyranosyl-(l-"6)]-β-D-glucopyranoside (7), diosgenin 3-O-{O-α-L-rhamnopyranosyl-(1→2)-O-[β-D-xylopyranosyl- (1→3)]-β-D-glucopyranoside} (8), (25R)-3β,17α-dihydroxy- 5α-spirostan-6-one-3-O-α-L-rhamnopyranosyl-(1→2) -β-D-glucopyranoside (9), (25R)-3β-hydroxy-5α-spirostan-6-one-3- O-α-L-rhamnopyranosyl-0 (1→2)-β-D-glucopyranoside (10), and (25R)-spirost-5-ene-3β-O-α-L-rhamnopyranosyl-(1→2) -[β-D-glucopyranosyl-(1→6)]-β-D-glucopyranoside (11). Compounds 1-5 showed the favorable scavenging effects on DPPH and ABTS. Conclusion: The study suggests that the phenylic constituents from the bulbs of L. lancifolium have the anti-oxidant effects.